vero cell infection medium  (Valiant Co Ltd)

Journal: Current protocols in stem cell biology

Article Title: FACS-Mediated Isolation of Neuronal Cell Populations From Virus-Infected Human Embryonic Stem Cell-Derived Cerebral Organoid Cultures.

doi: 10.1002/cpsc.65

Figure Lengend Snippet: Figure 2 Virus infection and fluorescence-assisted cell sorting. (A) Schematic overview of virus infection and sorting of cerebral organoid cultures. (B) Example of a plaque assay readout. Vero cells were infected with 10-fold serial dilutions as indicated. (C) Examples of cell sorting plots for isolating astrocytes, neurons, and neural progenitor cells from hESC-derived cerebral organoid cultures. (D) Illustration of ZIKV– and ZIKV+ FACS gating for all cells and sorted neural progenitors, astrocytes, and neurons.

Article Snippet: Materials VERO cells (ATCC CCL-81) Vero cell culture medium (see recipe) Zika virus strain, e.g., Uganda 1947 (MR 766, ATCC 1838) or Puerto Rico 2015 (PRVABC59, ATCC VR-1843) Vero cell infection medium (see recipe) 2× overlay medium (see recipe) 1% (w/v) DEAE dextran (MP Biomedicals, cat. no. 195133) in distilled water (sterilize by filtration) 5% (w/v) sodium bicarbonate (Fisher Scientific, cat. no. L-14639) in distilled water 2% Oxoid agar stock solution (see recipe) Janssens et al. 5 of 15 Current Protocols in Stem Cell Biology 19388969, 2019, 1, D ow nloaded from https://currentprotocols.onlinelibrary.w iley.com /doi/10.1002/cpsc.65 by U niversity O f T he Philipines D ilim an M ain L ibrary, W iley O nline L ibrary on [05/04/2024].

Techniques: Virus, Infection, FACS, Plaque Assay, Derivative Assay